|
KMID : 0895820030130040099
|
|
Journal of Oriental Rehabilitation Medicine
2003 Volume.13 No. 4 p.99 ~ p.109
|
|
|
Effects of Dokhwalgisaeng-tang (Duhuojisheng-tang) and Herbar Chelidoni on the Cultured Spinal Motor Neurons
|
|
Lee Young-Bo
Yun Sang-Hak
Lim Yang-Eui
Park Jin-Sang
Joung Taek-Sang
Han Kyung-Ho
Lee Su-Kyung
Shin Byung-Cheul
Kwon Young-Dal
Song Yung-Sun
|
|
|
Abstract
|
|
|
Objectives and Methods: To evaluate the mechanism of oxidative damage by Xanthine oxidase(XO) and hypoxanthine(HX)-induced oxygen radicals, XTT assay was carried out after the cultured mouse spinal motor neurons were preincubated for 5 hours with various concentrations of XO/HX. And DNA synthesis, PKC activity and c-fos immunohistochemistry were measured to evaluate the protective effect of Dokhwalgisaeng-tang(Duhuojisheng-tang: DGT), Dokhwalgisaeng-tanggami(Duhuojisheng-tangjiawei: DGTG), Herbar Chelidoni(HC) water extract on cultured spinal motor neurons damaged by XO/HX.
Results: The results were XO/HX showed the quantitative decrease of DNA synthesis, increase of PKC activity and c-fos positive cell, in proportion to concentration and exposed time on cultured spinal motor neurons and DGT, DGTG and HC showed the protective effects against decrease of DNA synthesis and increase of PKC activity and c-fos positive cell by XO/HX.
Conclusions: From the above results, it is suggested that XO/HX have a neurotoxic effect on cultured spinal motor neurons and the herbs extract, such as DGT, DGTG and HC, prevent the toxicity of XO/HX effectively.
|
|
|
KEYWORD
|
|
|
Dokhwalgisaeng-tang(Duhuojisheng-tang), XTT, DNA synthesis, PKC activity, c-fos positive cell
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|